Part:BBa_K4655005

Lysis Cassette

This is a lysis cassette gene from the genome of lambda phage.

This sequence contains 3 genes, (1)the S gene, which encodes a holin that punctures the cell membrane,(2)the R gene, which encodes a lysozyme that degrades peptidoglycan in the periplasm, and(3)the Rz-Rz1 gene, which encodes a spanin that destroys the inner and outer membrane. Expression of these genes causes E. coli to lyse.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

1. Gayan S. Abeysekera, Michael J. Love, Sarah H. Manners, Craig Billington, Renwick C. J. Dobson (2022). Bacteriophage-encoded lethal membrane disruptors: Advances in understanding and potential applications. Front. Microbiol., 26 October 2022 Sec. Phage Biology, Volume 13 - 2022

2.Gillian L. Ryan, Andrew D. Rutenberg (2007). Clocking Out: Modeling Phage-Induced Lysis of Escherichia coli. ASM Journals, Journal of Bacteriology, 1 July 2007, Vol. 189, No. 13.

3. Jesse Cahill and Ry Young (2019). Phage Lysis: Multiple Genes for Multiple Barriers. Adv Virus Res. 2019; 103: 33–70.

4. Christos G. Savva, Jill S. Dewey, Samir H. Moussa, Kam H. To, Andreas Holzenburg, Ry Young (2014). Stable micron-scale holes are a general feature of canonical holins. molecular microbiology Volume91, Issue1, January 2014, Pages 57-65